有氧健身操锻炼对普通女大学生STI的影响     

陈婷婷 《上海交通大学学报(农业科学版)》2013,(3):98-100

探讨较长时间的有氧健身操锻炼对普通女性大学生STI是否有良性的影响，30名普通女性大学生分成实验组（15人，3次/周， 90min/次）和对照组（15人，1次/周， 90min/次），共16周（一个学期）有氧健身操定量锻炼。分别在锻炼前、第8周末、16周结束时测量STI指标。结果表明，8周有氧健身操锻炼有一定的效果，但并不显著，直到16周结束STI相应指标才出现较明显的变化。提示：短期的有氧健身操锻炼对心脏的影响较小，要使心脏发生与运动相适应性的良性变化，锻炼的周期要长，且要保证每周锻炼的次数和时间。  相似文献   

Proteomic analysis of human endometrium during the implantation window     

TAN Zhen  LI Jie  LI Ming-tao  LIU Wei  YU Wen-juan  XIA Ting-ting 《园艺学报》2012,28(2):201-206

AIM: To establish a method for determining the differential expression of proteins in human endometrium during the implantation window and to analyze the correlation between altered expression of the proteins and endometrial receptivity. METHODS: A comparative proteomic strategy in a combination of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was adopted to search the proteomic alternations in the endometria of pre-receptive pre-receptive [day 2 after luteinizing hormone surge (LH+2 d)] state versus the endometria of receptive (LH+7 d) state. Validation of annexin IV was performed by Western blotting. RESULTS: Approximate 2 555±98 polypeptide spots were revealed by densitometry analysis of the 2D protein maps in LH+2 d and LH+7 d endometrial tissues resolved in the linear range of pH 3~10 on the 2D gel, in which 31 proteins were found to be significantly changed, including 17 proteins up-regulated and 14 proteins down-regulated in LH+7 d samples. These 31 identified proteins were classified into 6 functional categories of the correlation with implantation process: cell migration or assimilation, enzymic activity, signal transduction and gene regulation, immunoregulation, vascularization, and blood clotting or fibrinolysis system. The same expression trend of annexin IV was confirmed by Western blotting. CONCLUSION: Human endometrium has a differential proteomic repertoire during the window of implantation. The 6 functional categories of differentially expressed proteins in the receptive endometrium indicate that they play an important role in transforming of the endometrium during the receptive state.  相似文献   

Ontogeny of protease, amylase and lipase in the alimentary tract of hybrid Juvenile tilapia (Oreochromis niloticus × Oreochromis aureus)     

Li Jun-sheng  Li Jian-lin  Wu Ting-ting 《Fish physiology and biochemistry》2006,32(4):295-303

Juvenile tilapias (Oreochromis niloticus × O. aureus), with average body weights of groups I, II and III of 55.14, 122.82, and 225.68 g respectively were used to study changes in the activity of protease, amylase, and lipase in different organs and sections of the alimentary tract. Additionally, tract containing digesta, which is closer to physiological conditions, may help reveal the mechanisms of whole chemical digestion process. Another group of tilapias (mean body weight of 111.81 ± 0.14 g) was used to analyze changes in activity of protease, lipase, and amylase in response to varying pH and temperatures. The activity of enzymes in all digestive organs increased when body weight increased from 55 to 122 g, and the activity further increased when the weights increased from 122 to 225 g. The rates of increase in digestive enzyme activity were significantly greater than the rates of growth of the fish. When the activity was compared in different sections of the tract, a similar regularity was found for the three enzymes in all fish. The highest amounts of activity were seen in the foregut and the lowest in the rectum, with the middle amount of activity in the hindgut. The pH ranges for the maximum activity of tilapia protease in the stomach and intestine were 1.6–3.3 and 9.5–10.5 respectively and the optimal temperature in these organs was 55°C. The pH ranges for the maximum amylase activity in the intestine and hepatopancreas were 6–7 and 7.5 respectively, and high amylase activity in these organs was found at 25–35°C. The activity of lipase in the intestine remained unchanged at pH 6.0–9.0 and with temperatures between 25 and 35°C. These results suggest that the ability of tilapia to utilize feed varies at different growth stages and remains relatively stable with changes in environmental pH and temperature, which should be taken into account when designing feed formulas for tilapia.  相似文献   

The role of TF-1 cell apoptosis-related gene 19 in systemic lupus erythematosus     

SONG Qing-hua  WANG Jing  CHEN Ying-yu  DI Chun-hui  SUN Rong-hua  CHEN Xue-rong  LI Shi-yin 《园艺学报》2003,19(2):189-193

AIM:To clarify the role of new apoptosis-related gene, TF-1 cell apoptosis-related gene 19(TFAR19), in the pathogenesis of systemic lupus erythematosis (SLE) and the relationship between TFAR19 and SLE.METHODS:DNA Ladder detection, Western blotting, immunological fluorescence method, ELISA and so on were used to test if ultraviolet B(UVB) could induce HaCaT cell apoptosis and TFAR19 expression.RESULTS:HaCaT cell apoptosis could be detected after 24 hours of 30 mj/cm² UVB irradiation. Also, we found that in active SLE patients, the TFAR19 antibody was increased, but not significant compared to the normal control.CONCLUSION:TFAR 19 is involved in the process of UVB induced ketatinocyte line HaCaT apoptosis and SLE pathogenesis.  相似文献   

水分胁迫下华山松抗腐烂病的研究   总被引：1，自引：0，他引：1  

陈玉惠  谢开立等 《四川林业科技》2001,22(3):1-5

用不同浓度的聚乙二醇（PEG6000）对华山松幼苗进行诱导水分胁迫，树皮的相对膨胀度（RT值）随PEG浓度的增大而下降；且树皮内的酚类物质含量、多酚氧酶（PPO）活性和苯丙氨酸解氨酶（PAL）活性均有所降低，过氧化物酶（POD）活性升高。说明水分状况直接影响着华山松的抗病性。4种酚类物质抑菌试验表明，一定浓度下酚类物质对腐烂病病原菌松黑腐皮壳（Valsa pirni Fr.)菌丝体生长有抑制作用，以香草醛和邻苯二酚抑菌能力最强。  相似文献   

Nasopharyngeal cryptococcosis   总被引：1，自引：0，他引：1  

R MALIK  P MARTIN  DI WIGNEY  DB CHURCH  W BRADLEY  CR BELLENGER  WA LAMB  VR BARRS  S FOSTER  S HEMSLEY  PJ CANFIELD  DN LOVE 《Australian veterinary journal》1997,75(7):483-488

Naturally occurring cryptococcosis in five cats, a dog and a koala is described. Involvement of the nasopharynx was documented in all patients, and nasopharyngeal mass lesions accounted for the major presenting complaints in four. Signs referable to nasopharyngeal disease included snoring, stertor, inspiratory dyspnoea and aerophagia. Diagnoses were made by caudal rhinoscopy using a retroverted flexible endoscope, vigorous orthograde flushing with saline, or at necropsy. Concurrent cryptococcal rhinitis was present in all cases, although involvement appeared limited to the caudal nasal cavity in most cases. Typical signs of nasal cavity disease, such as sneezing and nasal discharge, were often absent. Treatment of nasopharyngeal cryptococcosis should include physical dislodgement or debulking of lesion(s) to provide immediate alleviation of upper airway obstruction, followed by systemic antifungal therapy to eliminate residual infection from the nasal cavity. Infections caused by Cryptococcus neoformans var gattii accounted for a disproportionately large number of these cases.  相似文献   

新疆吐鲁番地区葡萄根区土壤养分分析   总被引：2，自引：0，他引：2  

孔婷婷  岳朝阳  焦淑萍  张新平  刘爱华  杨健 《北方园艺》2015,(9):151-154

以葡萄根区土壤为试材,在吐鲁番地区具有代表性的"无核白"葡萄园内进行土样采集,测定了土壤中有机质和N、P、K、B、Fe、Zn、Mn、Cu等营养元素含量。结果表明:该地区葡萄根区土壤有机质含量偏低,全氮、速效氮处于缺乏状态,速效磷和速效钾含量丰富,微量元素除有效硼和有效铜含量处于较高水平外,铁、锌、锰含量均在不同程度上存在缺乏状况。表明吐鲁番地区葡萄园应增施有机肥,同时根据土壤质地、树龄等的不同,因地制宜,平衡施肥。  相似文献   

金银花和红银花挥发性成分的顶空固相微萃取#br# 气质联用检测与比较     

崔婷婷  单长松  吴澎  周涛 《园艺学报》2015,42(11):2283-2290

 采用顶空固相微萃取气质联用（SPME–GC–MS）的方法检测红银花与金银花新鲜花蕾和 干花蕾的挥发性成分。4 种花蕾样品共鉴定出67 种挥发性物质,其中新鲜红银花与金银花分别检测出48 种和46 种,经烘干处理的干红银花与金银花分别检测出34 种和37 种。[3.2.1.0（1,5）]三环辛烷、β– 芳樟醇、5–甲基–2–己醇和以及十六烷是4 种样品共有的成分。其中红银花挥发性成分主要以烃类、 醇酮类为主,β–芳樟醇含量显著高于金银花;样品在由新鲜到烘干这一过程中,烃类和酯类挥发性物质 含量降低,醛类和醇酮类挥发性物质显著增加。  相似文献   

Expression of Gab2 in human osteosarcoma U2-OS cells     

PING Yong  DI Ping-shan  LI Wei-bing  LI Feng-mei  CHEN Wei-yi  CHEN Ping-ping 《园艺学报》2015,31(3):568-571

AIM: To investigate the expression of Grb2-associated binding protein 2 (Gab2) in human osteosarcoma cells and its relationship with the invasion and metastases of human osteosarcoma cells. METHODS: The technique of small RNA interference was used to transfect human osteosarcoma U2-OS cell lines. Western blotting and RT-PCR were used to detect the protein and mRNA expression of Gab2 in transfected U2-OS cells. After transfection, through chemotaxis and invasion assays in vitro, the cell migration and invasion abilities were detected. RESULTS: After transfection, the expression of Gab2 at mRNA and protein levels in Gab2 siRNA transfected cells (SiGab2/U2-OS) was lower than that in scrambled siRNA transfected cells (Scr/U2-OS) and U2-OS cells. After stimulation with epidermal growth factor (EGF) at concentration of 10 μg/L, the migration SiGab2/U2-OS cells was significantly less than Scr/U2-OS cells and U2-OS cells (P<0.01). The number of invasion cells of SiGab2/U2-OS group was significantly lower than the other 2 control groups (P<0.01). CONCLUSION: Inhibition of Gab2 expression obviously attenuates the migration and invasion abilities of human osteosarcoma U2-OS cell line.  相似文献   

转基因抗虫棉Bt基因的遗传连锁分析   总被引：2，自引：0，他引：2  

肖松华  狄佳春  刘剑光  许乃银  陈旭升  黄骏麒 《棉花学报》2002,14(3):134-137

对中美两国所获得的转基因抗虫棉品种GK— 1 2、新棉 33B分别进行了 Bt基因的连锁测验。分析表明两个品种的试验结果相似 ,Bt基因未整合在棉花第 I、II、III、IV、V、VI、VII、IX、X、XI、XII、XIII、XVII连锁群及 cu、pg、gl1等基因所涉及的染色体上。阐明了 Bt基因染色体图谱定位的复杂性 ,并提出以原位杂交技术实现 Bt基因在棉花染色体组上的物理标图  相似文献